A phosphotyrosine-imprinted polymer receptor for the recognition of tyrosine phosphorylated peptides |
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Authors: | Emgenbroich Marco Borrelli Cristiana Shinde Sudhirkumar Lazraq Issam Vilela Filipe Hall Andrew J Oxelbark Joakim De Lorenzi Ersilia Courtois Julien Simanova Anna Verhage Jeroen Irgum Knut Karim Kal Sellergren Börje |
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Institution: | 1. INFU, Technische Universit?t Dortmund, Otto Hahn Strasse 6, 44221 Dortmund (Germany);2. Sunderland Pharmacy School, University of Sunderland, Sunderland SR1?3SD (UK);3. Department of Pharmaceutical Chemistry, University of Pavia, Via Taramelli 12, 27100 Pavia (Italy);4. Department of Chemistry, Ume? University, 90187 Ume? (Sweden);5. Cranfield Health, Cranfield University at Silsoe, Silsoe MK45?4DT (UK) |
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Abstract: | Hyperphosphorylation at tyrosine is commonly observed in tumor proteomes and, hence, specific phosphoproteins or phosphopeptides could serve as markers useful for cancer diagnostics and therapeutics. The analysis of such targets is, however, a challenging task, because of their commonly low abundance and the lack of robust and effective preconcentration techniques. As a robust alternative to the commonly used immunoaffinity techniques that rely on phosphotyrosine(pTyr)-specific antibodies, we have developed an epitope-imprinting strategy that leads to a synthetic pTyr-selective imprinted polymer receptor. The binding site incorporates two monourea ligands placed by preorganization around a pTyr dianion template. The tight binding site displayed good binding affinities for the pTyr template, in the range of that observed for corresponding antibodies, and a clear preference for pTyr over phosphoserine (pSer). In further analogy to the antibodies, the imprinted polymer was capable of capturing short tyrosine phosphorylated peptides in the presence of an excess of their non-phosphorylated counterparts or peptides phosphorylated at serine. |
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Keywords: | antibody mimics epitome imprinting molecular recognition phosphopeptides proteomics |
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