Chemical profiling and anti-psoriatic activity of methanolic extract of Andrographis nallamalayana J.L.Ellis |
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| Authors: | Sunitha Parlapally Neeraja Cherukupalli Sudarshana Reddy Bhumireddy Prabhakar Sripadi Ravindernath Anisetti Charu Chandra Giri |
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| Institution: | 1. Centre for Plant Molecular Biology, Osmania University, Hyderabad 500 007, India;2. Department of Pharmacy and Biotechnology, University College of Technology, Osmania University, Hyderabad 500 007, India;3. National Centre for Mass Spectrometry, CSIR-Indian Institute of Chemical Technology, Hyderabad 500 007 Telangana, India;4. Academy of Scientific and Innovative Research, CSIR-IICT, Hyderabad, India;5. Department of Pharmacy and Biotechnology, University College of Technology, Osmania University, Hyderabad 500 007, India |
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| Abstract: | Andrographis nallamalayana is being widely used as tribal medicine in the treatment of leucoderma and mouth ulcers. Chemical profiling of methanolic extract of the whole plant (PE), using GC–MS and LC–MS, revealed the presence of compounds viz. α-tocopherol, β-sitosterol, tetradecanoic acid, monostearin, flavones/flavanones and their glycosides, chromones, etc. Topical application of imiquimod on the dorsal portion of male BALB/C mice resulted in the development of psoriatic symptoms (erythema, scaling, thickening and folding) with a mean disease activity index (DAI) of >7.0. Topical treatment with 100-μL PE (~6.4%/12.8%) formulations, for 12-days, resulted in the alleviation of disease symptoms. Compared to water-based formulations, emu oil-based formulation, PE400EO was found more effective in reducing the mean DAI (>84%), keratinocyte count (>65%) (p < 0.01) and interleukin-22 (~70%) (p < 0.05). We report, for the first time, anti-psoriatic activity of A. nallamalayana having great potential in developing a potent phytomedicine against psoriasis. |
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| Keywords: | Andrographis nallamalayana chemical profiling antipsoriatic activity mouse model interleukin-22 |
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