A new coumarin isolated from Sarcandra glabra as potential anti-inflammatory agent |
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| Authors: | Maojie Wang Jin Zhao Ya Zhao Run-Yue Huang Geng Li |
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| Institution: | 1. Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China;2. The Postdoctoral Research Station, Guangzhou University of Chinese Medicine, Guangzhou, China;3. School of Chinese Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, China |
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| Abstract: | One new coumarin, 3,5-dihydroxy-7-O-α-L-rhamno pyranosyl-2H-chromen-2-one (1), was isolated from the whole plant of Sarcandra glabra. The structure was elucidated by spectroscopic methods. Our results indicated that 1 significantly inhibit nitric oxide (NO) production in LPS-induced RAW264.7 macrophages. RT-PCR analysis indicated it inhibited iNOS mRNA expression. In addition, Western blot analysis showed that 1 attenuated LPS-induced synthesis of iNOS protein in the macrophages. These results suggest that 1 could be potential anti-inflammatory agent by down-regulating iNOS expression. |
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| Keywords: | Sarcandra glabra coumarin RAW264 7 NO iNOS |
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