Isolation and identification of polyphenols from Marsilea quadrifolia with antioxidant properties in vitro and in vivo |
| |
Authors: | Yuan Zhang Hai-Yan Tian Ya-Fang Tan Yuk-Lau Wong Hoi Yan Wu Jun-Feng Jia |
| |
Affiliation: | 1. College of Pharmacy, Jinan University, Guangzhou 510632, P.R. China;2. State Key Laboratory of Phytochemistry and Plant Resources in West China (CUHK), Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, P.R. China;3. School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, P.R. China |
| |
Abstract: | Marsilea quadrifolia is an edible aquatic medicinal plant used as a traditional health food in Asia. Four new polyphenols including kaempferol 3-O-(2″-O-E-caffeoyl)-β-d-glucopyranoside (1), kaempferol 3-O-(3″-O-E-caffeoyl)-α-l-arabinopyranoside (3), 4-methy-3′-hydroxypsilotinin (4) and (±)-(E)-4b-methoxy-3b,5b-dihydroxyscirpusin A (18) together with 14 known ones (2, 5–17) were isolated from the ethanol extract of M. quadrifolia. Structures of the new compounds were elucidated by extensive spectroscopic analyses. In DPPH and oxygen radical absorbance capacity antioxidant assays, some compounds showed stronger antioxidant activities and quercetin (9) was the most potent antioxidant in both assays. In a restraint-induced oxidative stress model in mice, quercetin significantly attenuated the increase in plasma ALT and AST levels as well as liver MDA content of restrained mice. Liver SOD activity was also significantly increased by quercetin, indicating a significant in vivo antioxidant activity. As a rich source of polyphenols with strong antioxidant activities, M. quadrifolia may be developed to a product for relieving oxidative stress. |
| |
Keywords: | Marsilea quadrifolia polyphenol antioxidant activities DPPH ORAC restraint stress |
|
|