Antioxidant,antimicrobial and urease inhibiting activities of methanolic extracts from Cyphostemma digitatum stem and roots |
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Authors: | Rasool Khan Abdullah Qasem Saif Mohammed Mansour Quradha Jawad Ali Abdur Rauf Ajmal Khan |
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Affiliation: | 1. Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, KPK, Pakistanrasoolkhan1@hotmail.com;3. Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, KPK, Pakistan;4. Department of Chemistry, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan |
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Abstract: | Cyphostemma digitatum stem and roots extracts were investigated for antioxidant, antimicrobial, urease inhibition potential and phytochemical analysis. Phytochemical screening of the roots and stem extract revealed the presence of secondary metabolites including flavonoids, alkaloids, coumarins, saponins, terpenoids, tannins, carbohydrates/reducing sugars and phenolic compounds. The methanolic extracts of the roots displayed highest antioxidant activity (93.518%) against DPPH while the crude methanolic extract of the stem showed highest antioxidant activity (66.163%) at 100 μg/mL concentration. The methanolic extracts of both stem and roots were moderately active or even found to be less active against the selected bacterial and fungal strains (Tables S2 and S3). The roots extract (methanol) showed significant urease enzyme inhibition activity (IC50 = 41.2 ± 0.66; 0.2 mg/mL) while the stem extract was found moderately active (IC50 = 401.1 ± 0.58; 0.2 mg/mL) against thiourea (IC50 = 21.011; 0.2 mg/mL). |
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Keywords: | Cyphostemma digitatum roots and stem phytochemical screening antibacterial antifungal antioxidant enzyme inhibition activity |
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