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Determination of volatile organic compounds in the dried leaves of Salvia species by solid-phase microextraction coupled to gas chromatography mass spectrometry
Authors:Rosaria Cozzolino  Sadrollah Ramezani  Antonella Martignetti  Angela Mari  Sonia Piacente  Beatrice De Giulio
Institution:1. Institute of Food Science, National Council of Research, Avellino Italyrcozzolino@isa.cnr.it;3. Department of Horticulture Science, College of Agriculture, Tarbiat Modares University, Tehran, Iran;4. Institute of Food Science, National Council of Research, Avellino Italy;5. Department of Pharmacy, University of Salerno, Fisciano, Italy
Abstract:Salvia spp. are used throughout the world both for food and pharmaceutical purposes. In this study, a method involving headspace solid-phase microextraction combined with gas chromatography–mass spectrometry was developed, to establish the volatiles profile of dried leaves of four Iranian Salvia spp.: Salvia officinalis L., Salvia leriifolia Benth, Salvia macrosiphon Boiss. and two ecotypes of Salvia reuterana Boiss. A total of 95 volatiles were identified from the dried leaves of the five selected samples. Specifically, α-thujone was the main component of S. officinalis L. and S. macrosiphon Boiss. (34.40 and 17.84%, respectively) dried leaves, S. leriifolia Benth was dominated by β-pinene (27.03%), whereas α-terpinene was the major constituent of the two ecotypes of S. reuterana Boiss. (21.67 and 13.84%, respectively). These results suggested that the proposed method can be considered as a reliable technique for isolating volatiles from aromatic plants, and for plant differentiation based on the volatile metabolomic profile.
Keywords:Salvia  volatile organic compounds  solid-phase microextraction  GC–MS  metabolomic profile
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