pH-dependent cytotoxic effects of extracts of the marine sponge Polymastia janeirensis on cancer cell lines |
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| Authors: | Renata Biegelmeyer Rafael Schröder Douglas F Rambo Roger R Dresch João L F Carraro Beatriz Mothes B |
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| Institution: | 1. Pharmacognosy Laboratory, Faculty of Pharmacy, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazilrenata.biegel@gmail.com;3. Biochemistry Department, Study Center of Oxidative Stress, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil;4. Pharmacognosy Laboratory, Faculty of Pharmacy, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil;5. Department of Invertebrates, National Museum, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil;6. Zoobotanical Foundation, Museum of Natural Sciences, Porto Alegre, Brazil |
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| Abstract: | The purpose of this work was to study the cytotoxic effects of marine sponge Polymastia janeirensis, which has been observed in the field to release an orange substance that is toxic to fish. The result showed that aqueous extract (pH 7.0) was highly cytotoxic to glioma (U87) and neuroblastoma (SHSY5Y) cancer cell lines (IC50 < 1.0 μg/mL). In addition, this extract showed potent antioxidant and procoagulant (decreased the clotting time by 1.7-fold) activities. Interestingly, the cytotoxic effects were pH-dependent since the viability of the cancer cells was not affected with the extract (pH 5.5). The close similarity between the aqueous extract (pH 7.0) and the orange liquid that is released by the sponge indicates that this potential chemical defence of P. janeirensis deserves further investigation. |
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| Keywords: | Polymastia janeirensis chemical defence cytotoxic pH-dependent activity |
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