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Development of an HPLC post-column antioxidant assay for Solidago canadensis radical scavengers |
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| Authors: | Mindaugas Marksa Jolita Radušienė Valdas Jakštas Liudas Ivanauskas Rūta Marksienė |
| Affiliation: | 1. Department of Analytical and Toxicological Chemistry, Faculty of Pharmacy, Medical Academy of Lithuanian University of Health Sciences, Eiveni? g. 4, Kaunas 50161, Lithuaniamindaugas.m.lsmu@gmail.com;3. Institute of Botany, Nature Research Centre, Akademijos g. 2, Vilnius 08412, Lithuania;4. Department of Pharmacognosy, Faculty of Pharmacy, Medical Academy of Lithuanian University of Health Sciences, Eiveni? g. 4, Kaunas 50161, Lithuania;5. Department of Analytical and Toxicological Chemistry, Faculty of Pharmacy, Medical Academy of Lithuanian University of Health Sciences, Eiveni? g. 4, Kaunas 50161, Lithuania |
| Abstract: | The aim of this work was to modify and validate the post-column high-performance liquid chromatography (HPLC)-ABTS and DPPH methods for evaluating the antioxidant activity of the methanolic extracts of Solidago canadensis (Canadian goldenrod) leaves and flowers. Separation of the analytes was performed via the HPLC-PDA method on a YMC analytical column using a gradient elution program. Three compounds with antioxidant properties – chlorogenic acid, rutin and isoquercitrin – and two unidentified antioxidants were established. The research showed that the coil temperature regimes and loop length combinations influence the optimised post-column assay method for detecting the antioxidant activity of goldenrod radical scavengers. Investigations established that the temperature in the reaction coil was a substantial factor contributing to the signal strength of the analytes after reacting with the DPPH and ABTS radicals. |
| Keywords: | HPLC post-column coil temperature loop length DPPH ABTS Solidago canadensis |