Hydroxypropyl beta cyclodextrin bonded superficially porous particle-based HILIC stationary phases |
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| Authors: | Rasangi M Wimalasinghe Choyce A Weatherly Zachary S Breitbach |
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| Institution: | Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, Texas, USA |
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| Abstract: | (R,S)-Hydroxypropyl-modified β-cyclodextrin (RSP-CD) is a well-known chiral stationary phase. In this work, hydrophilic interaction liquid chromatographic (HILIC) selectivities of RSP-CD was demonstrated. Further, an evaluation of chromatographic performances of fully porous particles (FPPs)- and superficially porous particles (SPPs)-based RSP-CD stationary phases was performed. The RSP-CD-bonded SPP-based stationary phase showed faster and more efficient HILIC separations compared to the FPP-based stationary phases. In addition, the SPP-based RSP-CD stationary phase showed excellent selectivities for many classes of small polar molecules. Since the SPP-based stationary phase is allowed for separations performed at high flow rates without significant loss of efficiency, ultrafast separations (analysis times under 1 min) also was accomplished utilizing SPP-based RSP-CD stationary phase. |
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| Keywords: | Efficiency fast separations fully porous particles hydrophilic interaction liquid chromatography (R S)-hydroxypropyl-modified β-cyclodextrin superficially porous particles |
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