In vitro antifungal activity of extracts obtained from Hypericum perforatum adventitious roots cultured in a mist bioreactor against planktonic cells and biofilm of Malassezia furfur |
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Authors: | Giovanna Simonetti Noemi Tocci Alessio Valletta Elisa Brasili Felicia Diodata D'Auria Alicia Idoux |
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Institution: | 1. Department of Public Health and Infectious Diseases, Sapienza Università di Roma, Rome, Italy;2. Department of Environmental Biology, Sapienza Università di Roma, Rome, Italy;3. ROOTec Bioactives AG, Benkenstrasse 254, 4108Witterswil, Switzerland |
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Abstract: | Xanthone-rich extracts from Hypericum perforatum root cultures grown in a Mist Bioreactor as antifungal agents against Malassezia furfur.Extracts of Hypericum perforatum roots grown in a bioreactor showed activity against planktonic cells and biofilm of Malassezia furfur. Dried biomass, obtained from roots grown under controlled conditions in a ROOTec mist bioreactor, has been extracted with solvents of increasing polarity (i.e. chloroform, ethyl acetate and methanol). The methanolic fraction was the richest in xanthones (2.86 ± 0.43 mg g? 1 DW) as revealed by HPLC. The minimal inhibitory concentration of the methanol extract against M. furfur planktonic cells was 16 μg mL? 1. The inhibition percentage of biofilm formation, at a concentration of 16 μg mL? 1, ranged from 14% to 39%. The results show that H. perforatum root extracts could be used as new antifungal agents in the treatment of Malassezia infections. |
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Keywords: | antifungal activity biofilm bioreactor Hypericum perforatum Malassezia furfur root cultures xanthones |
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