A new bioactive diterpenoid from pestalotiopsis adusta,an endophytic fungus from clerodendrum canescens |
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Authors: | Ming-Feng Xu Ou-Ya Jia Sheng-Jia Wang |
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Affiliation: | Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, P.R. China |
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Abstract: | Bioassay-guided fractionation of the culture extract of Pestalotiopsis adusta, an endophytic fungus isolated from the medicinal plant Clerodendrum canescens, led to the isolation of one new, (10S)-12,16-epoxy-17(15→16)-abeo-3,5,8,12,15-abietapentaen-2,7,11,14-tetraone (1), and four known diterpenoids, teuvincenone F (2), uncinatone (3), coleon U (4), coleon U-12-methyl ether (5). These structures were identified by using spectroscopic methods, including UV, MS, 1D and 2D NMR experiments. This is the first report of these compounds being isolated from a Pestalotiopsis species. The cytotoxic activities of the compounds were evaluated, and compounds 1 and 3 demonstrated cytotoxic activities against the HL-60 tumour cell line (IC50 < 20 μM). |
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Keywords: | Pestalotiopsis adusta endophytic fungus abietane diterpenes cytotoxic activity |
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