Antileishmanial diterpenoid alkaloids from Aconitum spicatum (Bruhl) Stapf |
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| Authors: | Sajan L. Shyaula Tensangmu Tamang Nida Ghouri Achyut Adhikari Shanti Marasini Gan B. Bajracharya |
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| Affiliation: | 1. Faculty of Science, Nepal Academy of Science and Technology, Lalitpur, Nepalshyaulasajan@gmail.com;3. Department of Chemistry, Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal;4. HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan;5. Faculty of Science, Nepal Academy of Science and Technology, Lalitpur, Nepal |
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| Abstract: | The crude extracts of tubers of Aconitum spicatum (Bruhl) Stapf were investigated for in vitro antileishmanial activity against Leishmania major. The dichloromethane extract at pH 2.5 showed antileishmanial activity with IC50 value of 27.10 ± 0.0 μg/mL. Chromatographic purification of the dichloromethane extract led to isolation of three C-19 norditerpenoid alkaloids indaconitine (1), chasmaconitine (2) and ludaconitine (3). Compounds 3 and 2 showed antileishmanial activity with IC50 = 36.10 ± 3.4 and 56.30 ± 2.1 μg/mL, respectively. Compound 1 was less effective (IC50 > 100 μg/mL). The cytotoxicity of compounds 1, 2 and 3 studied against MCF7, HeLa and PC3 cancer cell lines and 3T3 normal fibroblast cell line did not show cytotoxicity at 30 μM. |
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| Keywords: | Aconitum spicatum antileishmanial activity Leishmania major diterpenoid alkaloids |
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