Isolation and Enrichment of Pathogens with a Surface‐Modified Aluminium Chip for Raman Spectroscopic Applications |
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Authors: | Susanne Pahlow Sandra Kloß Dr. Verena Blättel Konstantin Kirsch Dr. Uwe Hübner Dr. Dana Cialla Dr. Petra Rösch Dr. Karina Weber Prof. Dr. Jürgen Popp |
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Affiliation: | 1. Spectroscopy and Imaging, Institute of Photonic Technology, Albert‐Einstein‐Str. 9, 07745 Jena (Germany), Fax: (+49)?(0)3641?206?399;2. Institute of Physical Chemistry, Friedrich‐Schiller‐University Jena, Helmholtzweg 4, 07743 Jena (Germany);3. R‐Biopharm AG, An der neuen Bergstra?e 17, 64297 Darmstadt (Germany) |
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Abstract: | We developed a Raman‐compatible chip for isolating microorganisms from complex media. The isolation of bacteria is achieved by using antibodies as capture molecules. Due to the very specific interaction with the targets, this approach is promising for isolation of bacteria even from complex matrices such as body fluids. Our choice of capture molecules also enabled the investigation of samples containing yet unidentified bacteria, as the antibodies can capture a large variety of bacteria based on their analogue cell wall surface structures. The capability of our system is demonstrated for a broad range of different Gram‐positive and Gram‐negative germs. Subsequent identification is done by recording Raman spectra of the bacteria. Further, it is shown that classification with chemometric methods is possible. |
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Keywords: | aluminium antibodies biosensors Raman spectroscopy silanes |
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