Enzyme‐Controlled Sensing–Actuating Nanomachine Based on Janus Au–Mesoporous Silica Nanoparticles |
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| Authors: | Reynaldo Villalonga Paula Díez Alfredo Sánchez Elena Aznar Ramón Martínez‐Máñez José M Pingarrón |
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| Institution: | 1. Department of Analytical Chemistry, Complutense University of Madrid, 28040, Madrid (Spain), Fax: (+34)?913944329;2. IMDEA Nanoscience, Cantoblanco Universitary City, 28049, Madrid (Spain);3. Departamento de Química and CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER‐BBN), Universidad Politécnica de Valencia, Camino de Vera s/n, 46022, Valencia (Spain), Fax: (+34)?963879349;4. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Centro Mixto Universidad, Politécnica de Valencia‐Universidad de Valencia (Spain) |
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| Abstract: | Novel Janus nanoparticles with Au and mesoporous silica faces on opposite sides were prepared using a Pickering emulsion template with paraffin wax as the oil phase. These anisotropic colloids were employed as integrated sensing–actuating nanomachines for enzyme‐controlled stimuli‐responsive cargo delivery. As a proof of concept, we demonstrated the successful use of the Janus colloids for controlled delivery of tris(2,2’‐bipyridyl) ruthenium(II) chloride from the mesoporous silica face, which was grafted with pH‐sensitive gatelike scaffoldings. The release was mediated by the on‐demand catalytic decomposition of urea by urease, which was covalently immobilized on the Au face. |
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| Keywords: | controlled release enzyme Janus nanoparticle mesoporous silica molecular gates |
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