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Preparation and characterization of monodisperse superparamagnetic poly(vinyl alcohol) beads by reverse spray suspension crosslinking
Authors:Chengli Yang  Yueping Guan  Jianmin Xing  Guobin Shan  Huizhou Liu
Institution:1. Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, P.O. Box 353, China;2. Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057;3. School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China
Abstract:A novel protocol for preparing magnetic poly(vinyl alcohol) (PVA) beads by reverse spray suspension crosslinking was reported. The hydrophilic Fe3O4 nanoparticles were mixed with PVA, glutaraldehyde, and water to form aqueous phase. Then the aqueous phase was sprayed into vegetable oil by a pressure of nitrogen gas to form water in oil (W/O) suspension. The magnetic PVA beads were obtained in the presence of hydrochloric acid catalyst. It was found that the magnetic PVA beads obtained good properties when the PVA concentration was 10%, and the oil phase temperature was controlled at 40 °C. The mechanical stirring has little impact on the size of magnetic PVA beads in the process of reverse spray suspension crosslinking. The Cibacron Blue (CB) was coupled on the surface of magnetic PVA beads by surface chemical reaction. The morphology, size, and magnetic properties of the magnetic PVA beads were examined by scanning electron microscopy, laser diffraction, and vibrating sample magnetometer, respectively. Compared with the stirring method, it was found that the size of magnetic PVA beads was monodisperse and their saturation magnetization was much higher. Fourier transform infrared and X‐ray photoelectron spectroscopy experimental results proved that CB molecules were covalently immobilized onto the surface of the magnetic PVA beads. Meanwhile, the protein affinity separation experiments demonstrated that the magnetic PVA beads can potentially be used as a carrier for large‐scale protein separation. © 2007 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 46: 203–210, 2008
Keywords:biodegradable  crosslinking  magnetic polymers  poly(vinyl alcohol) beads  protein separation  superparamagnetic
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