Adsorption kinetics of proteins onto solid surfaces in the limit of the interfacial interaction control |
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| Affiliation: | 1. CTR Odontologiques, INSERM U 157, 1, place de l''Hôpital, 67000 Strasbourg France;2. Institut Charles SADRON (CRM-EAHP), CNRS-ULP Strasbourg, 6, rue Boussingault, 67083 Strasbourg Cedex France;1. Department of Physics, Sri Sathya Sai Institute of Higher Learning (SSSIHL), PSN Campus, Puttaparthi, A.P, 515134, India;2. Department of Chemistry, SSSIHL, India;3. Department of Biosciences, SSSIHL, India;4. Optical Imaging and Integration Laboratory, Central Research Instruments Facility, SSSIHL, India;5. IFN-CNR CSMFO Lab., FBK Photonics Unit, Povo-Trento, Italy;6. Museo Storico della Fisica e Centro Studi e Ricerche “Enrico Fermi”, Piazza del Viminale 1, 00184, Roma, Italy;7. FabULLAS, FemtoFab, Central Research Instruments Facility, SSSIHL, India;1. imec, Kapeldreef 75, 3001 Leuven, Belgium;2. KU Leuven-University of Leuven, Department of Physics and Astronomy, 3001 Leuven, Belgium;3. KU Leuven-University of Leuven, Centre of Surface Chemistry and Catalysis, 3001 Leuven, Belgium;1. Institute of Carbon Neutrality and New Energy, School of Electronics and Information, Hangzhou Dianzi University, Hangzhou 310018, PR China;2. Zhejiang Energy Technology Co., Ltd., Hangzhou 310023, PR China;3. College of Materials and Environmental Engineering, Hangzhou Dianzi University, Xiasha Campus, Hangzhou 310018, PR China;1. Analytical Chemistry – Center for Electrochemical Sciences (CES), Faculty of Chemistry and Biochemistry, Ruhr University Bochum, Universitätsstr. 150, D-44780 Bochum, Germany;2. Center for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia, L.go Rosanna Benzi 10, I-16132 Genoa, Italy;3. Department of General Zoology and Neurobiology, Faculty of Biology and Biotechnology, Ruhr University Bochum, Universitätsstr. 150, D-44780 Bochum, Germany;1. School of Chemistry, University of New South Wales, Sydney, NSW 2052, Australia;2. Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, NSW 2232, Australia |
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| Abstract: | We have developed an experimental set-up using radiolabelled proteins for the continuous measurement, as a function of time, of the excess concentration of superficial protein at a solid/liquid interface. The experimental conditions were designed in order to minimize the coupling of the interfacial interaction with bulk diffusion, and therefore to work within the limit of the interfacial interaction control. Chemical kinetics were assumed to follow a Langmuir equation. The absorption (Ka) and desorption (Kd) rate constants have been evaluated in the case of fibrinogen and albumin adsorption, onto glass beads and synthetic hydroxyapatite powder, respectively. In order to determine these parameters, a non-linear differential equation (obtained by combining the Langmuir rate equation and a mass-balance equation) was solved by the fourth-order Runge-Kutta method. The results obtained are consistent with the hypothesis of an adsorption rate controlled by the interfacial interaction. |
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