Comparative study between a commercial and a homemade capillary electrophoresis instrument for the simultaneous determination of aminated compounds by induced fluorescence detection |
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Authors: | Silvia Casado-Terrones Sonia Cortacero-Ramírez Alegría Carrasco-Pancorbo Antonio Segura-Carretero Alberto Fernández-Gutiérrez |
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Institution: | (1) Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda. Fuentenueva s/n, 18071 Granada, Spain |
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Abstract: | The performance of two capillary electrophoresis (CE) instruments, one commercial and one homemade device, were compared for
the determination of derivatised aminated compounds with fluorescein isothiocyanate (FITC). The commercial CE system first
uses an argon ion laser as excitation source; the homemade CE device uses an inexpensive blue-light-emitting diode (LED) as
the light source and a charge-coupled device (CCD) as the detection system. After fine optimisation of several separation
parameters in both devices, a co-electroosmotic flow CE methodology was achieved in coated capillary tubing with 0.001% hexadimetrine
bromide (HDB), and 50 mmol L−1 sodium borate at pH 9.3 with 20% 2-propanol for the determination of several amines and aminoacids. Analytical performances,
applicability in beer samples and other aspects such as cost or potential for miniaturization have been compared for both
devices. |
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Keywords: | Capillary electrophoresis Laser-induced fluorescence Light-emitting diode (LED)-induced fluorescence Aminated compounds Co-electroosmotic flow |
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