| EXPRESSION OF A PARTIAL SYNTHETIC HUMAN TNF cDNA IN E. coli |
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| 作者姓名： | 李昌本 柴常星 谢毅 吕群 柴建华 俞红 陈春妹 林丽珠 王启松 李宗林 周亚 李育阳 赵寿元 |
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| 作者单位： | State Key Laboratory of Genetic Engineering，Institute of Geneics，Fudan University，Shanghai 200433，PRC，State Key Laboratory of Genetic Engineering，Institute of Geneics，Fudan University，Shanghai 200433，PRC，State Key Laboratory of Genetic Engineering，Institute of Geneics，Fudan University，Shanghai 200433，PRC，State Key Laboratory of Genetic Engineering，Institute of Geneics，Fudan University，Shanghai 200433，PRC，State Key Laboratory of Genetic Engineering，Institute of Geneics，Fudan University，Shanghai 200433，PRC，State Key Laboratory of Genetic Engineering，Institute of Geneics，Fudan University，Shanghai 200433，PRC，State Key Laboratory of Genetic Engineering，Institute of Geneics，Fudan University，Shanghai 200433，PRC，State Key Laboratory of Genetic Engineering，Institute of Geneics，Fudan University，Shanghai 200433，PRC，State Key Laboratory of Genetic Engineering，Institute of Geneics，Fudan University，Shanghai 200433，PRC，State Key Laboratory of Genetic Engineering，Institute of Geneics，Fudan University，Shanghai 200433，PRC，State Key Laboratory of Genetic Engineering，Institute of Geneics，Fudan University，Shanghai 200433，PRC，State Key Laboratory of Genetic Engineering，Institute of Geneics，Fudan University，Shanghai 200433，PRC，State Key Laboratory of Genetic Engineering，Institute of Geneics，Fudan University，Shanghai 200433，PRC |
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| 摘 要： | A recombinant human tumour necrosis factor (rhTNF) cDNA was constructed. The TNF gene was isolated from a human genomic gene library. There are four exons in the TNF gene. The fourth exou codes for 140 amino acids of the TNF matured protein which is composed of 157 amino acids. A major portion of the fourth exon was isolated and then ligated to a synthesized DNA fragment coding for the remaining amino acids. The partial synthetic hTNF (rhTNF) cDNA thus generated was subcloned into a vector and successfully expressed in E. coli. 5-1 fer1entator was used to produce rhTNF. About 20g (wet weight) of bacterial pellet per liter medium and 106—10~7 units of cytotoxicity to L929 cells per milliliter medium were obtained. rhTNF was purified by HPLC and dried with a freeze dryer, rhTNF with a purity of about 95% in the form of white powder was obtained. The sequence of ten amino acids at the amino terminus of the rhTNF was determined. The result showed that it was identical with that of the natural human TNF.
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EXPRESSION OF A PARTIAL SYNTHETIC HUMAN TNF cDNA IN E. coli |
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| LI CHANG-BEN CHAI CHANG-XING XIE YI L QUN CHAI JIAN-HUA YU HONG CHEN CHUN-MEI LIN LI-ZHU WANG QI-SONG LI ZONG-LIN ZHOU YA LI YU-YANG ZHAO SHOU-YUAN.EXPRESSION OF A PARTIAL SYNTHETIC HUMAN TNF cDNA IN E. coli[J].Science in China(Chemistry),1992(3). |
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| Authors: | LI CHANG-BEN CHAI CHANG-XING XIE YI L QUN CHAI JIAN-HUA YU HONG CHEN CHUN-MEI LIN LI-ZHU WANG QI-SONG LI ZONG-LIN ZHOU YA LI YU-YANG ZHAO SHOU-YUAN |
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| Abstract: | |
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| Keywords: | recombinant human tumout necrosis factor (thTNF) cDNA eytotoxieity of TNF rhTNF expression in E coil |
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