Determination of tetrodotoxin and its analogs in the puffer fish Takifugu oblongus from Bangladesh by hydrophilic interaction chromatography and mass-spectrometric detection |
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| Authors: | Marc Diener Bernd Christian M. Sagir Ahmed Bernd Luckas |
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| Affiliation: | (1) Institute of Nutrition, University of Jena, Dornburger Street 25, 07743 Jena, Germany;(2) Laboratory of Aquatic Resource Management, Department of Zoology, University of Dhaka, Dhaka, 1000, Bangladesh |
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| Abstract: | Tetrodotoxin (TTX) and its analogs (TTXs), widely distributed among marine as well as terrestrial animals, induce dangerous intoxications. These highly potential toxins are also known as the causative agent of puffer fish poisoning. A newly developed highly sensitive method for determination of TTXs based on hydrophilic interaction chromatography and mass-spectrometric detection is presented. TTX, anhydrotetrodotoxin, 11-deoxytetrodotoxin and trideoxytetrodotoxin were determined in separated tissues of Bangladeshi marine puffers, Takifugu oblongus. TTX was predominant in skin, muscle and liver, whereas trideoxytetrodotoxin preponderated in the ovary. The toxicity of the various tissues was determined by a mouse bioassay. |
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| Keywords: | Hydrophilic interaction chromatography Liquid chromatography– tandem mass spectrometry Takifugu oblongus Tetrodotoxin Zwitterionic hydrophilic interaction chromatography column |
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