Isoelectric focusing sample injection for capillary zone electrophoresis in a fused silica capillary. |
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| Authors: | Luo-Hong Zhang Cheng-Jie Zhang Xing-Zheng Wu |
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| Affiliation: | Department of Materials Science and Engineering, Faculty of Engineering, University of Fukui, Bunkyo 3-9-1. Fukui 910-8507, Japan. |
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| Abstract: | An improvement has been made to couple isoelectric focusing (IEF) sample injection and capillary zone electrophoresis in an untreated fused silica capillary. Electroosmotic flow is efficiently prevented by simply using a rubber block at the outlet end of the capillary during IEF sample injection. The experimental conditions that affect the concentration effect are discussed. A concentration enhancement factor of over 100-fold can be easily obtained for two model proteins: lysozyme and ribonuclease A. |
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