基于网状混合自组装膜的压电免疫传感器及其应用

结合纳米金及混合自组装技术, 制备了一种新型网状混合膜, 提出了一种新的生物分子固定化方法, 研制了一种用于检测人血清抗精子抗体的压电免疫传感器. 首先, 将纳米金溶胶、巯基丙酸和1,6-二巯基己烷按一定的比例混合制得网状混合自组装膜, 然后将此膜组装到压电石英晶振的金电极表面, 经EDC/NHS活化后, 再将抗原固定到电极上, 实现对抗精子抗体的检测. 结果表明, 该方法能明显提高抗体抗原结合效率, 从而提高传感器的灵敏度, 并降低传感界面的非特异性吸附. 将此传感器应用于人血清抗精子抗体的检测, 线性范围为10~800 mU/mL, 检出限为7 mU/mL. 此传感器为抗精子抗体的临床检测提供了新平台.

Piezoelectric Immunosensor Based on a Network-type Mixed Self-assembled Monolayer and Its Application

An immunosensor for the analysis of antisperm antibody in human serum was developed. The detection methodology is based on the network-type mixed thin film on gold electrode surface. The network-type mixed thin film was derived by a one-step mixed-assembly-cross-linking route. In this strategy, the mixing of 1,6-hexananedithiol, 3-mercaptopropionic acid and Au nanoparticles in solutions allowed sequential co-assemble, cross-linked and eventual precipitation of the network-type mixed thin film on gold electrode, which yielded an interface containing carboxyl groups for immobilization of sperm antigens. The immunoresponse of the proposed immunosensor was compared with that based on mixed self-assembled monolayer immobilized on plane electrode. It was found that the developed sensing interface could perform more effective antibody-antigen binding and decrease nonspecific adsorption. This immunosensor can quantitatively determine concentrations of antisperm antibody ranging from 10 to 800 mU/mL with a detection limit of 7 mU/mL. Five human serum samples were determined with proposed immunosensor and the results were in good agreement with those obtained by ELISA method, which means the piezoelectric immunosensor could provide a new platform for the clinic detection of antisperm antibody.