| 在线多通道拉曼光谱检测系统的建立及与毛细管电泳的联用 |
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| 引用本文： | 李忠,王玉洁,刘国诠,陈义. 在线多通道拉曼光谱检测系统的建立及与毛细管电泳的联用[J]. 高等学校化学学报, 2001, 22(10): 1654-1657 |
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| 作者姓名： | 李忠 王玉洁 刘国诠 陈义 |
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| 作者单位： | 中国科学院化学研究所分子科学中心, 北京 100080 |
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| 基金项目： | 国家杰出青年基金 (批准号 :2 982 5 112 )，中国科学院重大基金 (批准号 :KJ95 1-A1-5 0 7)资助 |
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| 摘 要： | 利用氩离子激光器和ICCD自组装了毛细管电泳-激光拉曼光谱联用分析系统,用已知拉曼谱图的苯和甲苯对系统进行调试,详细讨论了外光路系统对灵敏度的影响.用甲基橙和甲基红混合体系进行拉曼-毛细管电泳联用的实验研究,得到了较好的甲基橙和甲基红的在线电泳-时间分辨激光拉曼谱图.
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| 关 键 词： | 拉曼光谱 毛细管电泳 在线联用 甲基红 甲基橙 |
| 文章编号： | 0251-0790(2001)10-1654-04 |
| 收稿时间： | 2000-10-19 |
| 修稿时间： | 2000-10-19 |
The Establishment of On-line Multichannel Raman Spectroscopic Detection System for Capillary Zone Electrophoresis |
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| LI Zhong,WANG Yu-Jie,LIU Guo-Quan,CHEN Yi. The Establishment of On-line Multichannel Raman Spectroscopic Detection System for Capillary Zone Electrophoresis[J]. Chemical Research In Chinese Universities, 2001, 22(10): 1654-1657 |
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| Authors: | LI Zhong WANG Yu-Jie LIU Guo-Quan CHEN Yi |
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| Affiliation: | Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China |
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| Abstract: | An on|line multichannel Raman spectroscopic detection system for capillary electrophoresis was established with an Ar++ laser and a cryogenically cooled ICCD as the key elements. Its exterior light system was carefully adjusted to optimize the detection by using benzene and toluene as the probes. On|line electrophoretogram and time series of enhanced Raman spectra of methyl red and methyl orange were acquired at 1 mmol/L and 488 nm laser light for excitation. |
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| Keywords: | Raman spectroscopy Capillary electrophoresis On|line coupling Methyl red Methyl orange |
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