Chemical constituents and protective effect of Ficus ingens (Miq.) Miq. on carbon tetrachloride-induced acute liver damage in male Wistar albino rats |
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| Authors: | Abd El Raheim M Donia Gamal A Soliman Ahmed M Zaghloul Saleh I Alqasoumi Amani S Awaad Asmaa M Radwan Omer A Basodan |
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| Institution: | 1. Pharmacognosy Department, College of Pharmacy, Salman Bin Abdulaziz University, Al-Kharj, Saudi Arabia;2. Medicinal and Aromatic Plants Department, Desert Research Center, Cairo, Egypt;3. Pharmacology Department, College of Pharmacy, Salman Bin Abdulaziz University, Al-Kharj, Saudi Arabia;4. Pharmacognosy Department, College of Pharmacy, Mansoura University, Egypt;5. Pharmacognosy Department, College of Pharmacy, King Saud University, Saudi Arabia;6. Chemistry Department, College of Science, King Saud University, Saudi Arabia;7. Botany and Microbiology Department, College of Science, Girls Branch, Al Azhar University, Cairo, Egypt |
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| Abstract: | The aim of the present study was to investigate the chemical constituents and hepatoprotective effect of Ficus ingens (Miq.) Miq. (Moraceae) extract against carbon tetrachloride-induced acute liver damage in male Wistar albino rats. The ethanol extract of F. ingens, was subjected to phytochemical study. In addition, its acute and sub-chronic toxicities were assessed. Eight compounds were isolated from this plant and identified as β-sitosterol, β-sitosterol glucoside, chryasophanol, 7-hydroxy-2,5 dimethyl chromen-4-one, quercetin, Aloe emodin glucoside, rutin and Patuletin-3′-O-methyl-3-O-rutinoside. The structure elucidation was based on 1H and 13C NMR, proton–proton correlation spectroscopy (1H–1H Cosy), distortionless enhancement by polarization transfer (DEPT), Heteronuclear Multiple-Quantum Correlation (HMQC), and heteronuclear multiple bond correlations spectrum (HMBC). Hepatotoxicity induced with CCl4 was evidenced by elevation of liver marker enzymes (ALT, AST, ALP and LDH) and TB content in serum. In addition, antioxidant enzymes were drastically inhibited with significant reduction of GSH and increased LPO in liver homogenate of CCl4-intoxicated rats. Pre-treatment with F. ingens (200 and 400 mg/kg) and silymarin (50 mg/kg) avoided the changes observed in CCl4-intoxicated rats. In conclusion, the ethanol extract of F. ingens showed protective activity against liver injury, which might be developed into a new hepatoprotective agent. |
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| Keywords: | Phytochemical studies Flavonoids Antharquinone Hepatoprotective effect |
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