Isolation of the cytoplasmatic 5S ribosomal RNA from cotton seeds by preparative electrophoresis

Summary 1. The complete separation of low-molecular-weight 5S rRNA and tRNA from fractions of high-molecular-weight rRNAs (28S + 18S) in a preparation of the total rRNA from cotton seeds has been achieved.2. The optimum conditions have been selected for the preparative electrophoresis and isolation of homogeneous preparations of 5S rRNA and total tRNA.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 489–494, July–August, 1978.