| 复合酶酶解法提取褐藻糖胶及其结构分析和抗氧化性研究 |
| |
| 引用本文： | 崔艳丽,王培培,周军明,张松嵩,毛建卫.复合酶酶解法提取褐藻糖胶及其结构分析和抗氧化性研究[J].浙江大学学报(理学版),2011,38(5):536-540. |
| |
| 作者姓名： | 崔艳丽 王培培 周军明 张松嵩 毛建卫 |
| |
| 作者单位： | 1. 浙江大学化学系,浙江杭州,310027
2. 浙江科技学院生物与化学工程学院,浙江杭州310023;浙江省农产品化学与生物加工技术重点实验室,浙江杭州310023 |
| |
| 基金项目： | 国家自然科学基金资助项目(30870553); 浙江省重点科技计划项目(2005C22037) |
| |
| 摘 要： | 用正交试验设计法研究了复合酶酶解法提取褐藻糖胶,使用sevage法脱蛋白,经DEAE-Sepharose FF离子交换层析后得到2个组分F1和F2,并分析了2个褐藻糖胶组分的结构,探讨了褐藻糖胶的抗氧化活性.优化提取工艺中褐藻糖胶的多糖质量分数是30.24%,硫酸根质量分数是21.25%.
|
| 关 键 词： | 褐藻糖胶 复合酶酶解 抗氧化 结构分析 |
Extraction of fucoidan using multienzyme enzymolysis and their structural analysis,antioxidation research |
| |
| CUI Yan-li,WANG Pei-pei,ZHOU Jun-ming,ZHANG Song-song,MAO Jian-wei.Extraction of fucoidan using multienzyme enzymolysis and their structural analysis,antioxidation research[J].Journal of Zhejiang University(Sciences Edition),2011,38(5):536-540. |
| |
| Authors: | CUI Yan-li WANG Pei-pei ZHOU Jun-ming ZHANG Song-song MAO Jian-wei |
| |
| Institution: | CUI Yan-li 1,WANG Pei-pei 1,ZHOU Jun-ming 1,ZHANG Song-song 1,MAO Jian-wei 2,3(1.Department of Chemistry,Zhejiang University,Hangzhou 310027,China,2.Department of Biological and Chemical Engineering,Zhejiang University of Science and Technology,Hangzhou 310023,3.Zhejiang Provincial Key Lab for Chem.& Bio.Processing Technology of Farm Produces,China) |
| |
| Abstract: | Fucoidan was isolated from the brown seaweed by multienzyme enzymolysis method,and the method was optimized by orthogonal experimental design.After removing the protein in the crude fucoidan using Sevage method,we got two components from crude fucoidan by DEAE-Sepharose FF ion exchange chromatogram,and analyzed their structure and antioxidation.The total sulfate and polysaccharide reached 21.25% and 30.24% respectively. |
| |
| Keywords: | fucoidan multienzyme enzymolysis antioxidation structural analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《浙江大学学报(理学版)》浏览原始摘要信息 |
| 点击此处可从《浙江大学学报(理学版)》下载免费的PDF全文 |
|
