DNA detection on lateral flow test strips: enhanced signal sensitivity using LNA-conjugated gold nanoparticles |
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Authors: | Rastogi Shiva K Gibson CharLene M Branen Josh R Aston D Eric Branen A Larry Hrdlicka Patrick J |
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Affiliation: | Biosensors and Nanotechnology Applications Laboratory, University of Idaho, Coeur d'Alene, ID 83814-2277, USA. srastogi@uidaho.edu |
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Abstract: | A lateral flow test strip assay, enabling sensitive detection of DNA specific to the foodborne pathogen E. coli O157:H7, is described. The use of LNA-conjugated gold nanoparticle probes, along with signal amplification protocols, results in minimum detectable concentrations of ~0.4 nM. |
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