Triacylglycerol lipase from rape (Brassica napus L.) suitable for biotechnological purposes |
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Authors: | Matthew J Hills Kumar D Mukherjee |
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Institution: | (1) Bundesanstalt für Fettforschung, Institut für Biochemie und Technologie, H. P. Kaufmann-Institut, Piusallee 68, D-4400 Münster, Germany |
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Abstract: | Triacylglycerol lipase (EC 3.1.1.3) from rape (Brassica napus L. cv Ceres) is quite easily prepared from the 100,000 x g supernatant of cotyledon homogenates. The lipase is present in
a high-molecular-mass fraction ( > 1.5 x 106 dalton by gel filtration), but it can be rapidly extracted from the 100,000 xg supernatant by precipitation with polyethyleneglycol
8000 (4%, w/v) and MgCl2 (40 mM) giving about a 10-fold purification. After delipidation, the lipase has an Mr of about 300,000. It hydrolyzes triacylglycerols to fatty acids and glycerol, although the fatty acids from the sn-1 or -3
positions are hydrolyzed first to yield 1,2(2,3)-diaclyglycerols. Lipase immobilized onto Celite by precipitation with acetone
at-20°C catalyzes the esterification of oleic acid with butanol dissolved in hexane. |
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Keywords: | Lipase fromBrassica napus immobilized enzyme esterification |
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