Absolute fluorescence quantum yields by relative fluorescence and photoacoustic measurements of low level luminescence quenching
Authors:
Joseph E. Sabol
Affiliation:
Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN 55455 U.S.A.
Department of Chemistry, Oklahoma State University, Stillwater, OK 74078, U.S.A.
Abstract:
A method is reported which allows absolute fluorescence quantum yields ΦF to be obtained from relative fluorescence and non-radiative emission (photoacoustic) data. Absolute calibration procedures, external standards, and total fluorescence quenching are eliminated through use of low concentrations of a heavy atom quencher. The relative decrease in fluorescence emission and increase in non-radiative emission as a function of the quencher concentration are related to ΦF through a set of simultaneous equations. Fluorescence quantum yields of ΦF = 0.59 ± 0.03 for cresyl violet perchlorate in EtOH and ΦF = 0.55 ± 0.02 for quinine sulfate in 0.1 N H2SO4 are reported.