Comparative study of epithelial and fibroblastic cell lines as an alternative cytotoxicity test to the Draize method |
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Authors: | Pinto T J de Azevedo J C Cruz A S |
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Affiliation: | Universidade de S?o Paulo, Faculdade de Ciências Farmacêuticas, Departamento de Farmácia, Brazil. |
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Abstract: | Several methods are being used with considerable advantage as alternatives to the Draize test, although some technical difficulties still persist. This work compared the sensitivity of HeLa and NCTC L 929 cells to evaluate the cytotoxicity of shampoos used by adults and children (undiluted and diluted to 25, 5, 1, and 0.1%), and eye drops and their containers and surfactants (diluted to 30, 10, 1, and 0.1%). Nondiluted adult shampoos and their 25 and 5% dilutions were cytotoxic for both cell lines. When diluted to 1%, only one of the shampoos was noncytotoxic, whereas among those diluted to 0.1%, only one was cytotoxic. Children's shampoos were cytotoxic when not diluted or diluted to 25%. From those diluted to 5%, only one was noncytotoxic for both cell lines. The cytotoxic tests showed that the eye drops and their containers were noncytotoxic. Surfactants were cytotoxic when diluted to 30 and 10% and noncytotoxic when diluted to 1 and 0.1%. An excellent correlation (r = 0.95) was demonstrated between the sensitivity of the HeLa and NCTC L929 cells in the evaluation of cytotoxicity reactions. |
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