Cryopreservation of Prunus avium L. embryogenic tissues |
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Authors: | Grenier-de March Ghislaine de Boucaud Marie-Therese Chmielarz Pawel |
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Institution: | Institut Superieur d'Agriculture de Beauvais (ISAB), Laboratoire de Biotechnologies Vegetales, Rue Pierre Waguet - BP 30313, 60026 Beauvais Cedex, France. ghislaine.grenier@isab.fr |
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Abstract: | Embryogenic tissues from wild cherry (Prunus avium L.) were successfully cryopreserved by using a one-step freezing procedure. Cryoprotection consisted of a pretreatment on solid medium with increasing sucrose concentrations (0.25 M for 1 day, 0.5 M for 1 day, 0.75 M for 2 days, and 1.0 M for 3 days), followed by air desiccation to about 20 percent moisture content (fresh weight basis). This method was compared with a pretreatment on solid medium containing 5 percent DMSO and 2 percent proline, followed by immersion in a modified PVS2 cryoprotective solution. Pretreatment on solid medium with increasing concentrations of sucrose led to regrowth of frozen embryogenic tissues, and after 6 weeks of culture, growth was comparable to that of non-dehydrated and non-frozen tissues. By contrast, no regrowth was observed when embryogenic tissues were submitted to the solid/liquid pretreatment with DMSO/proline and a modified PVS2 solution. |
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