| 硫堇-牛血清白蛋白氧化还原复合膜修饰葡萄糖生物传感器的研究 |
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| 引用本文： | 梁克中.硫堇-牛血清白蛋白氧化还原复合膜修饰葡萄糖生物传感器的研究[J].分析试验室,2011,30(3):58-62. |
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| 作者姓名： | 梁克中 |
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| 作者单位： | 重庆三峡学院化学与环境工程学院,万州,404100 |
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| 基金项目： | 重庆市教育委员会科研项目 |
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| 摘 要： | 通过交联法和自组装法制备了一种双酶型葡萄糖生物传感器.首先以牛血清白蛋白-戊二醛为交联剂以实现对辣根过氧化物酶(HRP)的固载,再利用凝集素-糖蛋白的识别作用将葡萄糖氧化酶(GOD)分子组装到电极表面,制得双酶型的葡萄糖生物传感器.采用原子力显微镜(AFM)考察了复合膜的性质,同时采用循环伏安法和计时电流法考察了该传感...
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| 关 键 词： | 牛血清白蛋白 硫堇 辣根过氧化氢酶 葡萄糖氧化酶 生物传感器 |
A bienzymic biosensor based on thionine-the bovine serum albumin redox composite membrane for the detection of glucose |
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| HANG Ke-zhong.A bienzymic biosensor based on thionine-the bovine serum albumin redox composite membrane for the detection of glucose[J].Chinese Journal of Analysis Laboratory,2011,30(3):58-62. |
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| Authors: | HANG Ke-zhong |
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| Institution: | LIANG Ke-zhong(College of Chemistry and Environment,Chongqing Three Gorges University,Wanzhou 400400) |
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| Abstract: | A bienzymic biosensor was prepared by means of cross-linking and self-assembly.Firstly,a solution of bovine serum albumin(BSA) containing horseradish peroxidase(HRP) is coated on the gold electrode in such a way that the first enzyme membrane is achieved.Then,by the use of sugar-lectin biospecific interactions,the glucose oxidase(GOD) was attached on the electrode surface between concanavalin A(Con A) and sugar chains intrinsically.The morphologies of composite films were characterized by atomic force micro... |
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| Keywords: | Bovine serum albumin Thionine Horseradish peroxidase Glucose oxidase Biosensor |
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