A new selective reagent for the spectrofluorometric determination of beryllium |
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| Authors: | V. Drevenkar Z. Štefanac A. Brbot |
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| Affiliation: | 1. Institute for Medical Research, Yugoslav Academy of Sciences and Arts, Mo?e Pijade 158, 41000 Zagreb, Yugoslavia;2. Laboratory of Organic Chemistry and Technology, Biochemistry Department, Faculty of Technology, University of Zagreb, 41000 Zagreb, Yugoslavia |
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| Abstract: | The fluorescence properties of the beryllium and aluminum complexes with 2, 4-dioxo-4-(4-hydroxy-6-methyl-2-pyrone-3-yl) butyric acid ethyl ester (Ligand) were studied and optimal conditions for their fluorometric determination were established. Beryllium can be determined in the linearity range of 0.5–2.0 μg/ml and aluminum 0.5–1.5 μg/ml. The effect of diverse ions on the determination of beryllium is discussed.A simple procedure for the fluorometric determination of beryllium in human blood plasma in the concentration range of 5–50 μg Be/ml is described. |
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