流动注射光度法测定黄酒中蛋白质的含量和分子量分布

将流动注射技术引入Bradford蛋白质测定法，利用Sepadex G-100凝胶层析柱分离黄酒中的蛋白质，可直接测定蛋白质的含量和分子量分布。成品酒（熟酒）为5．32g/L（6．86g／L）；高分子蛋白质（Fw〉25000）占10．52％（13、08％）；中分子蛋白质（FW：25000～5000）占49、12％（54．12％）；小分子蛋白质（FW〈5000）占40、36％（32、80％），并得到黄酒中蛋白质含量与分子量分布的曲线谱图，可监控和预测黄酒的品质。

Determination of Contents and Molecular Weights Distribution of Rice Wine Proteins by Flow Injection-Spectrophotometry

Rice wine was directly introduced to a Sepadex G-100 gel chromatography column,and rice wine proteins were eluted from the column and assayed by bradford method connected a flow injection analysis.The contents and molecular weights distribution of rice wine proteins are as follows:for the bottled rice wine,the protein content is 5.32 g/L and the fractins of hgith molecular weights(FW>25000),middle molecular weights(FW 25000~5000) and low molecular weights(FW<5000) are 10.52%,49.12% and 40.36%,(respectively).Compare with the bottled rice wine,those of mature rice wine are 6.86 g/L,13.08%,54.12% and 32.80%,respectively.The relation ship between contents and molecular weights distribution of rice wine protein was showed by an eluted curve.According to the eluted curve of rice wine protein,a series of profiles of rice wine proteins,was set up.It can be applied to evaluate and forecast the qualities of rice wine.