Determination of adapalene (CD271/differin®) and retinol in plasma and tissue by on-line solid-phase extraction and HPLC analysis |
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Authors: | R Rühl H Nau |
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Institution: | 1. Institut für Toxikologie und Embryopharmakologie, Fachbereich Humanmedizin, Freie Universit?t Berlin, Garystr. 5, 14195, Berlin, Germany
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Abstract: | Summary Adapalene, the active constituent of Differin?, is a novel potent retinoid (vitamin A analogue) for the topical treatment of acne vulgaris. The clinical usefulness of retinoids
is limited by a number of side effects, such as teratogenicity and skin irritation. A method has been developed for simultaneous
determination of adapalene and retinol in plasma and tissue in in vivo and in vitro studies for the determination of the pharmacokinetic
profile and the influence of adapalene on the endogenous retinol level. The new method was developed by coupling an autosampler
to an automated solid-phase extraction unit on-line with a gradient HPLC system using UV and fluorescence detection. The low
detection limit (0.25 ng mL−1 for adapalene), the small sample weight (50 mg) and the high degree of automation make this method convenient for analysis
of biological samples in animal and human studies.
Presented at the 21st ISC held in Stuttgart, Germany, 15th–20th September, 1996. |
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Keywords: | Column liquid chromatography Solid-phase extraction Differin Retinol Adapalene |
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