Production of a novel pyranose 2-0xidase by basidiomyceteTrametes multicolor |
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Authors: | Leitner Christian Haltrich Dietmar Nidetzky Bernd Prillinger Hansjörg Kulbe Klaus D |
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Institution: | 1.Abteilung Biochemiscbe Technologie, Institut für Lebensmitteltechnologie, Austria ;2.Lnstitut für Angewandte Mikrobiologie, Universit?t für Bodenkultur BOKU Wien (University of Agricultural Sciences Vienna), Muthgasse 18, A-1 190, Wien, Austria ; |
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Abstract: | During a screening for the enzyme pyranose 2-oxidase (P2O) which has a great potential as a biocatalyst for carbohydrate transformations,
Trametes multicolor was identified as a promising, not-yet-described producer of this particular enzyme activity. Furthermore,
it was found in this screening that the enzyme frequently occurs in basidiomycetes. Intracellular P2O was produced in a growth-associated
manner by T. multicolor during growth on various substrates, including mono-, oligo-, and polysaccharides. Highest levels
of this enzyme activity were formed when lactose or whey were used as substrates. Peptones from casein and other casein hydrolysates
were found to be the most favorable nitrogen sources for the formation of P2O. By applying an appropriate feeding strategy
for the substrate lactose, which ensured an elevated concentration of the carbon source during the entire cultivation, levels
of P2O activity obtained in laboratory fermentations, as well as the productivity of these bioprocess experiments, could be
enhanced more than 2.5-fold. |
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Keywords: | Index Entries" target="_blank">Index Entries rametes multicolor yranose 2-oxidase creening ulture medium development |
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