Determination of Synthetic Phenolic Antioxidants in Vegetable Oil and Oil-Enriched Foods by High-Performance Liquid Chromatography with Electrochemical Detection |
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| Authors: | Zheng Sun Meng Wang Ni Cheng Hui Gao Jianjun Deng Jing Zhao |
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| Institution: | 1. Department of Food Science and Engineering, School of Chemical Engineering, Northwest University, Xi’an, Shaanxi, China;2. Institute of Analytical Science, Shaanxi Provincial Key Lab of Electroanalytical Chemistry, Northwest University, Xi’an, Shaanxi, China |
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| Abstract: | Three synthetic phenolic antioxidants, tertiary butyl hydroquinone, butylated hydroxytoluene, and butylated hydroxyanisole, were determined in vegetable oil and oil-enriched food by high-performance liquid chromatography (HPLC) with electrochemical detection. The separation was achieved using a reverse-phase column and gradient elution with methanol and 1% acetic acid. The limits of detection and quantification of the analytes were 2–120 lower, higher than those obtained by diode-array detection. The recoveries were 103.3% for tertiary butyl hydroquinone, 97.3% for butylated hydroxyanisole, and 95.2% for butylated hydroxytoluene. The results showed that HPLC with electrochemical detection is suitable for the quantification of low concentrations of phenolic antioxidants in vegetable oil and oil-enriched food with high sensitivity and accuracy. |
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| Keywords: | Electrochemical detection high-performance liquid chromatography oil-enriched food synthetic phenolic antioxidants |
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