Chemical sensing of Benzo[a]pyrene using Corchorus depressus fluorescent flavonoids |
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Authors: | Wajiha Ahmad Sundus Riaz Nasir Mehmood Ahmad Maryam Hameed Ayesha Naeem |
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Affiliation: | 1. Department of Biomedical Engineering and Sciences, School of Mechanical and Manufacturing Engineering, National University of Science &2. Technology, Islamabad, Pakistan |
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Abstract: | Plant phytochemicals, such as flavonoids are in use for the development of optical biosensor. Benzo[a]pyrene (B[a]P), is a pervasive environmental and dietary carcinogen. A fluorescent assay is developed using plant isolated flavonoid for the detection of B[a]P. High content saponins are excluded from the flavonoid-containing methanolic extract of Corchorus depressus by implying reduction of silver ions by saponins resulting in formation of silver nanoparticles. Isolated plant flavonoids are used to develop a spectrofluorometric assay for the detection of B[a]P. Decrease in the flavonoid fluorescence intensity by B[a]P is found to be based on both static and dynamic quenching. Specificity of the assay for B[a]P was tested for other carcinogens belonging to different classes of compounds. Flavonoids-mediated sensing can be implied for the development of new generation of nanoparticle-based biosensors that can be more sensitive and less susceptible to external factors, such as temperature and humidity. |
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Keywords: | Benzo[a]pyrene Corchorus depressus fluorescence quenching flavonoids |
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