|
|||||
|
|
Monolithic rod columns for HPLC based on divinylbenzene-styrene copolymer with 1-vinylimidazole and 4-vinylpyridine |
|
| Authors: | Yuri Patrushev Yulia Yudina Vladimir Sidelnikov |
| Affiliation: | 1. Department of Physicochemical Methods, Boreskov Institute of Catalysis, Novosibirsk, Russia;2. Department of Natural Sciences, Novosibirsk State University, Novosibirsk, Russiapatrush@catalysis.ru;4. Department of Natural Sciences, Novosibirsk State University, Novosibirsk, Russia |
| Abstract: | Monolithic chromatographic columns for HPLC based on divinylbenzene-styrene both with 1-vinylimidazole and with 4-vinylpyridine are prepared. The monoliths were synthesized in glass tubes with the inner diameter of 2?mm. Texture, hydrodynamic and chromatographic properties of the prepared columns were studied. Linear solvation energy relationships model was applied for the characterization of columns selectivity It is shown that changing the on 1-vinylimidazole or 4-vinylpyridine content in the initial solution allows to change the selectivity of the columns. An examples of small molecules and some proteins separations are presented. |
| Keywords: | 1-vinylimidazole 4-vinylpyridine HPLC monolithic columns porous polymers |