Occurrence of aflatoxin M1 in milk samples from Italy analysed by online-SPE UHPLC-MS/MS |
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| Authors: | Luca Campone Anna Lisa Piccinelli Rita Celano Imma Pagano Rosa Di Sanzo Sonia Carabetta |
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| Affiliation: | 1. Department of Agricolture, Università Mediterranea di Reggio Calabria, Reggio Calabria, Italy;2. Department of Pharmacy, Università degli studi di Salerno, Fisciano, Italylcampone@unisa.it;4. Department of Pharmacy, Università degli studi di Salerno, Fisciano, Italy |
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| Abstract: | The occurrence of aflatoxin M1 in 69 milk samples collected in a south region of Italy in 2016 was evaluated. The samples were analysed using an automated method based on online SPE coupled with UHPLC tandem mass spectrometry. After a salt induced liquid–liquid extraction with acetonitrile to remove protein from milk, the extract was diluted with water and analysed using an automated online SPE MS/MS method. Among the analysed samples no one had AFM1 higher than the legally allowable limits whereas 71.4% of the other analysed samples were above the LOD of the method. The highest contamination level of AFM1 was found in pasteurised milk (44.39 ng kg?1). The results show the worrying and widespread of AFM1 contamination, highlighting the necessity of monitoring studies in order to evaluate the reduction of the maximum legal limit. |
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| Keywords: | Aflatoxin M1 mycotoxin analysis on-line SPE occurrence of aflatoxin M1 milk and milk samples |
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