Identification and determination of naturally occurring folates in grains of rice (Oryza sativa L.) by UPLC-MS/MS analysis |
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| Authors: | Kaliyaperumal Ashokkumar Paramasivam Sivakumar Muthukrishnan Saradhadevi |
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| Institution: | 1. Department of Plant Biotechnology, School of Agriculture, PRIST University, Thanjavur, India;2. Department of Plant Sciences, University of Saskatchewan, Saskatchewan, Canadabiotech.ashok@gmail.com;4. Department of Plant Biotechnology, Agricultural College and Research Institute, Thanjavur, India;5. Department of Biochemistry, Bharathiar University, Coimbatore, India |
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| Abstract: | The genetic potential and biofortification of India-grown rice with bioavailable folate has not been studied yet. The objectives of this study were to determine the folates concentration in four cultivars of rice through UPLC–MS/MS. Total folate concentration in rice cultivars ranged from 11.0 to 51 μg/100 g with a mean of 26.0 μg/100 g. Among the four rice cultivars, the pigmented grain cultivar Nootripathu possesses two-fold rich sources of total folates than the other three non-pigmented grain cultivars. The average value of 100 g serving of rice grains could provide the amount of recommended daily allowance (% RDA) of dietary folates (6.5%) for adults, which ranged from 2.7–12.7%. Among the 5 individual forms of folates, 5-methyltetrahydrofolate was most abundant in rice cultivars followed by 10-Formylfolic acid and folic acid. The result of this study has been useful for biofortification of folates in rice. |
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| Keywords: | Rice folate profile food analysis food composition UPLC–MS/MS |
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