Determination of 4-Hydroxyphenyllactic Acid in Human Urine by Magnetic Solid-Phase Extraction and High-Performance Anion-Exchange Chromatography with Fluorescence Detection |
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Authors: | Junjun Liu Aiping Yan Yongli Yang |
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Affiliation: | 1. College of Chemistry, Nanchang University, Nanchang, Jiangxi, China;2. Center of Analysis and Testing, Nanchang University, Nanchang, Jiangxi, China |
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Abstract: | A novel, simple, rapid, and accurate method is reported for the determination of 4-hydroxyphenyllactic acid in human urine by high-performance anion-exchange chromatography with fluorescence detection and magnetic solid-phase extraction. The separation and pretreatment conditions for urine were optimized. The isolation of 4-hydroxyphenyllactic acid was performed with isocratic elution with 4?mmol?L?1 sodium hydroxide at 0.45?mL min?1. Fluorescence detection was performed at an excitation wavelength of 277?nm and an emission wavelength of 340?nm. Under the optimized conditions, the linear dynamic range and the limit of detection for 4-hydroxyphenyllactic acid were 0.05–10 and 0.020?mg?L?1, respectively. The recovery for the analyte was from 86.5 to 105.5%, with relative standard derivations less than 4.12%. The method was used for the determination of 4-hydroxyphenyllactic acid in human urine. Statistically significant differences in the 4-hydroxyphenyllactic acid concentration in urine were obtained between healthy control and individuals with breast cancer. |
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Keywords: | Fluorescence 4-hydroxyphenyllactic acid high-performance anion-exchange chromatography human urine magnetic solid-phase extraction. |
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