Anti-influenza effect of the major flavonoids from Salvia plebeia R.Br. via inhibition of influenza H1N1 virus neuraminidase |
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Authors: | Sunghee Bang Wei Li Thi Kim Quy Ha Changyeol Lee Won Keun Oh |
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Institution: | 1. College of Pharmacy, Duksung Women’s University, Seoul, Republic of Korea;2. Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu, Republic of Korea;3. College of Pharmacy, Seoul National University, Seoul, Republic of Korea |
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Abstract: | To determine the compounds responsible for its anti-influenza activities, we isolated the three flavonoids, 6-hydroxyluteolin 7-O-β-d-glucoside (1), nepitrin (2), homoplantaginin (3) from the MeOH extract of Salvia plebeia R.Br. and identified them by comparing the spectroscopic data with that reported in the literature. The contents of the three flavonoids in the whole extract were 108.74 ± 0.95, 46.26 ± 2.19, and 69.35 ± 1.22 mg/g for 6-hydroxyluteolin 7-O-β-d-glucoside, nepitrin, and homoplantaginin, respectively, which demonstrates that they are the major constituents of this plant. The three flavonoids were evaluated for their inhibitory activities against influenza virus H1N1 A/PR/9/34 neuraminidase and H1N1-induced cytopathic effect (CPE) on Madin-Darby canine kidney (MDCK) cells. Our results demonstrated the following arrangement for their anti-influenza activities: nepitrin (2) > 6-hydroxyluteolin 7-O-β-d-glucoside (1) > homoplantaginin (3). The potent inhibitory activities of these flavonoids against influenza suggested their potential to be developed as novel anti-influenza drugs in the future. |
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Keywords: | Influenza Salvia plebeia R Br flavonoid HPLC neuraminidase |
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