Lipase-catalyzed enantioselective transesterification of 3-hydroxy-3-(2-thienyl) propanenitrile in liquid carbon dioxide |
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| Authors: | Jiaxin Zhang Yazhuo Li Wenwei Qian Liu Zhang Fengxi Li |
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| Institution: | 1. Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, People’s Republic of China;2. College of Food Science and Engineering, Jilin University, Changchun, People’s Republic of China |
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| Abstract: | The transesterification of 3-hydroxy-3-(2-thienyl) propanenitrile catalyzed by Pseudomonas fluorescens lipase (PFL) in liquid carbon dioxide (CO2) was reported. Compared with that in organic solvent (n-hexane), the catalytic performance of PFL was dramatically enhanced in liquid CO2. Under the optimal reaction conditions, PFL exhibited an excellent enantioselectivity (E-value: 92.9) with a high enzyme activity (82.5?μmol/g/min). Besides, the remained (S)-3-hydroxy-3-(2-thienyl) propanenitrile with high enantiomeric purity (ee?>?99%) was obtained in 4?h when the conversion was about 52%.Lipase-catalyzed transesterification of 3-hydroxy-3-(2-thienyl) propanenitrile in liquid CO2 |
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| Keywords: | Lipase transesterification 3-hydroxy-3-(2-thienyl) propanenitrile liquid carbon dioxide |
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