Chemical constituents from Gueldenstaedtia verna and their anti-inflammatory activity |
| |
| Authors: | Xin Liu Chengle Yin Yue Cao Jinge Zhou Tao Wu |
| |
| Institution: | 1. Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai, China;2. Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, The Shanghai Key Laboratory for Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China |
| |
| Abstract: | A new triterpenoid glycoside (1) and 15 known compounds (2–16) were isolated from the whole plants of Gueldenstaedtia verna. The new compound (1) was identified as complogenin 22-O-β-d-glucopyranoside by extensive spectroscopic techniques including 1D (1H and 13C) and 2D NMR experiments (HSQC, HMBC and NOESY), HR-DART-MS and chemical methods. Most of the isolates were evaluated for their inhibitory activities on LPS-induced NO production in RAW 264.7 cells. The inhibitory effects of the active compounds, sulphuretin (8) and (22E,24S)-5α,8α-epidioxy-24-methyl-cholesta-6,9(11),22-trien-3β-ol (13), on the production of pro-inflammatory mediators (including IL-6, IL1β and PGE2) were further estimated in vitro by ELISA in RAW 264.7 macrophages. |
| |
| Keywords: | Gueldenstaedtia verna triterpenoid glycoside anti-inflammatory inhibition of NO production |
|
|
