Determination of Tetracyclines in Milk by Graphene-Based Solid-Phase Extraction and High-Performance Liquid Chromatography |
| |
Authors: | Xia Jun Wu Geng Nan Wang Kun Yang Hui Zhi Liu |
| |
Institution: | College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei, China |
| |
Abstract: | A graphene-based solid-phase extraction (SPE) column was prepared for the isolation of tetracyclines from milk followed by determination by high-performance liquid chromatography. Graphene provided better separation for tetracyclines than amine-modified graphene and carboxyl-modified graphene. The optimized graphene-based SPE column showed high absorption capacities (greater than 4,660?ng) and high recoveries (exceeding 92%) for tetracycline, oxytetracycline, chlortetracycline, and doxycycline and was successfully reused at least fifty times. The limits of detection in milk were from 10 to 20?ng/mL, with recoveries between 82.3 and 103.6%. Furthermore, the system showed superior performance than two commercial SPE cartridges with respect to recovery, purification, and reusability. Therefore, this approach is suitable for the determination of tetracyclines in milk. |
| |
Keywords: | Graphene high-performance liquid chromatography milk solid-phase extraction tetracyclines |
|
|