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Determination of Antibiotics in Surface Water by Solid-Phase Extraction and High-Performance Liquid Chromatography with Diode Array and Mass Spectrometry Detection |
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| Authors: | Maria-Loredana Soran Ocsana Opriş Veronica Floare-Avram Cristian Coman |
| Institution: | 1. Physics of Nanostructured Systems Department, National Institute for Research and Development of Isotopic and Molecular Technologies, Cluj-Napoca, Romania;2. Department of Mass Spectrometry, Chromatography and Applied Physics, National Institute for Research and Development of Isotopic and Molecular Technologies, Cluj-Napoca, Romania;3. Department of Taxonomy and Ecology, National Institute of Research and Development for Biological Sciences, Bucharest, Institute of Biological Research, Cluj-Napoca, Romania |
| Abstract: | A high-performance liquid chromatography method is reported for the determination of antibiotics in water. The antibiotics were simultaneously preconcentrated by solid-phase extraction. High-performance liquid chromatography was performed on a C18 modified column with gradient elution in 25?min at 40°C. The separation was performed using gradient elution with 90:10 acetonitrile:water and 0.1% aqueous formic acid. The antibiotics were identified by diode array detection and mass spectrometry. The established method was suitable for the determination of antibiotics in surface water. |
| Keywords: | Antibiotics high-performance liquid chromatography solid-phase extraction surface water |