Determination of lignans,phenolic acids and antioxidant capacity in transformed hairy root culture of Linum usitatissimum |
| |
Authors: | Ahmed M. M. Gabr Hoda B. Mabrok Emam A. Abdel-Rahim Mohamed K. El-Bahr Iryna Smetanska |
| |
Affiliation: | 1. Department of Plant Biotechnology, Genetic Engineering &2. Biotechnology Division, National Research Centre (NRC), Cairo, Egypt;3. Agricultural Faculty, Department of Plant Food Processing, University of Applied Science Weihenstephan-Triesdorf, Weidenbach, Germanya_m_gabr2@yahoo.com;5. Department of Nutrition and Food Science, Food industry and Nutrition Division, National Research Centre (NRC), Cairo, Egypt;6. Faculty of Agriculture, Department of Biochemistry, Cairo University, Cairo, Egypt;7. Agricultural Faculty, Department of Plant Food Processing, University of Applied Science Weihenstephan-Triesdorf, Weidenbach, Germany |
| |
Abstract: | Hairy root culture is a promising alternative method for the production of secondary metabolites. In this study, transformed root of Linum usitatissimum was established using Agrobacterium rhizogenes A4 strain from root cultures for lignans, phenolic acids and antioxidant capacity determination. Total lignin content (secoisolariciresinol diglucoside, secoisolariciresinol and matairesinol) was 55.5% higher in transformed root cultures than in the non-transformed root culture. Secoisolariciresinol was detected in higher concentration (2.107 μmol/g DM) in the transformed root culture than non-transformed culture (1.099 μmol/g DM). Secoisolariciresinol diglucoside and matairesinol were exclusively detected in the transformed root culture, but were not found in the non-transformed root culture. The overall production of phenolic acids in transformed roots was approximately 3.5 times higher than that of the corresponding non-transformed culture. Free radical scavenging DPPH˙ and ABTS˙+ assays showed 2.9-fold and 1.76-fold higher anti-oxidant activity in transformed root culture as compared to non-transformed. |
| |
Keywords: | Linum usitatissimum hairy root culture Agrobacterium rhizogenes lignans phenolic acids antioxidant capacity |
|
|