Proliferative and antioxidant activity of Symphytum officinale root extract |
| |
| Authors: | Ireneusz Sowa Roman Paduch Maciej Strzemski Sylwia Zielińska Ewelina Rydzik-Strzemska Jan Sawicki |
| |
| Affiliation: | 1. Department of Analytical Chemistry, Medical University of Lublin, Lublin, Poland;2. Faculty of Biology and Biotechnology, Department of Virology and Immunology, Maria Curie-Sklodowska University, Lublin, Poland;3. Department of General Ophthalmology, Medical University of Lublin, Lublin, Poland;4. Department of Pharmaceutical Biology, Wroclaw Medical University, Wroclaw, Poland |
| |
| Abstract: | The root of Symphytum officinale L. is commonly used in folk medicine to promote the wound healing, reduce the inflammation and in the treatment of broken bones. The objective of our investigation was to analyse the extract from S. officinale in term of its antioxidant activity and the effect on cell viability and proliferation of human skin fibroblast (HSF). Moreover, the quantification of main phenolics and allantoin was conducted using HPLC–DAD method. Five compounds were found: rosmarinic, p-hydroxybenzoic, caffeic, chlorogenic and p-coumaric acid. DPPH, FRAP and TPC assay showed the high antioxidant activity of the extract. MTT test proved the stimulatory effect on cell metabolism and viability of HSF cells. Moreover, no changes in cytoskeleton structure and cells shape were observed. The obtained results indicate that non-toxic extract from S. officinale root has strong antioxidant potential and a beneficial effect on human skin fibroblasts. |
| |
| Keywords: | Symphytum officinale comfrey cell viability human skin fibroblast antioxidant activity phenolic acids allantoin |
|
|
