Antioxidant potential assessment of phenolic and flavonoid rich fractions of Clematis orientalis and Clematis ispahanica (Ranunculaceae) |
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| Authors: | Ehsan Karimi Meisam Habibi Mahdi Ebrahimi Ali Mehrafarin Farahnaz Khalighi-Sigaroodi |
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| Affiliation: | 1. Faculty of Science, Department of Biochemistry and Biophysics, Islamic Azad University, Mashhad Branch, Mashhad, Iran;2. Faculty of Science and Engineering, Department of Biology, Gonbad Kavous University, Gonbad Kavous, Iran;3. Faculty of Veterinary Medicine, Department of Veterinary Preclinical Sciences, Universiti Putra Malaysia, Serdang, Malaysia;4. Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran |
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| Abstract: | The antioxidant activities of crude extract fractions using Hexane, Chloroform, Ethyl Acetate, Butanol and Water of Clematis orientalis and Clematis ispahanica were investigated. 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay and the ferric reducing/antioxidant potential (FRAP) were used to evaluate the antioxidant capacity. The total phenolics were found to be 4.37–9.38 and 1.32–11.37 mg gallic acid equivalents (GAE)/g in different fractions for C. orientalis and C. ispahanica, respectively. The ethyl acetate fraction of C. orientalis and chloroform fraction of C. ispahanica showed the highest DPPH and FRAP activities at a concentration of 300 μg/mL. The predominant phenolic compounds identified by HPLC in C. orientalis were Resorcinol (603.5 μg/g DW) in chloroform fraction and Ellagic acid (811.7 μg/g DW) in chloroform fraction of C. ispahanica. |
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| Keywords: | Clematis orientalis Clematis ispahanica antioxidant activity HPLC |
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