Cytotoxicity,In vitro anti-Leishmanial and fingerprint HPLC- photodiode array analysis of the roots of Trillium govanianum |
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| Authors: | Kashif Maqbool Khan Lutfun Nahar Abdul Mannan Ihsan Ul-Haq Muhammad Arfan Ghazanfar Ali Khan |
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| Institution: | 1. Faculty of Science, School of Pharmacy and Biomolecular Sciences, Medicinal Chemistry and Natural Products Research Group, Liverpool John Moores University, Liverpool, England, UK;2. Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad, Pakistan;3. Institute of Pharmaceutical Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistankashifmkhan007@gmail.com kashif.maqbool@uvas.edu.pk;5. Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad, Pakistan;6. Department of Pharmacy, Quaid-i-Azam University, Islamabad, Pakistan;7. Department of Chemistry, School of Natural Sciences, National University of Science and Technology, Islamabad, Pakistan;8. Department of Research and Development, Drug Regulatory Authority, Islamabad, Pakistan |
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| Abstract: | Trillium govanianum Wall. ex D. Don (Melanthiaceae alt. Trilliaceae), commonly known as ‘nagchhatry’ or ‘teen patra’, distributed from Pakistan to Bhutan about 2500–3800 m altitude is indigenous to Himalayas region. In folk medicine the plant has been reported for the treatment of wound healing, sepsis and in various sexual disorders. This paper reports, for the first time, to evaluate the cytotoxicity, in vitro anti-leishmanial (promastigotes) and fingerprint HPLC-photodiode array analysis of the MeOH extract of the roots of T. govanianum and its solid phase extraction fractions. Reverse phase HPLC-PDA based quantification revealed the presence of significant amount of quercetin, myrecetin and kaemferol ranging from 0.221to 0.528 μg/mg DW. MeOH extract revealed distinguishable protein kinase inhibitory activity against Streptomyces 85E strain with 18 mm bald phenotype. The remarkable toxicity profile against brine shrimps and leishmanial was manifested by MeOH extract with LC50 10 and 38.5 μg/mL, respectively. |
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| Keywords: | Trillium govanianum HPLC-PDA SPE protein kinase inhibition antileishmanial assay LC50 |
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